![]() ![]() This review provides an overview of distinct multiplex genome editing techniques employed in the engineering of such strains. cerevisiae strains, thus enabling ethanol production from cellulosic biomass. To tackle this hurdle, multiplex genome editing technologies offer a promising avenue for engineering recombinant cellulolytic S. ![]() Nonetheless, the production of cellulase enzymes in S. cerevisiae, the primary species employed for industrial ethanol production, exhibits stress tolerance and the ability to ferment diverse sugars from various feedstocks. However, the recalcitrance of cellulose poses a challenge to converting it into fermentable sugars, impeding the economic viability of bioethanol production. The production of biofuels from cellulosic biomass has emerged as an appealing alternative to fossil fuels. ![]()
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